Fiber-optic singlet oxygen [1O2 (1Delta(g))] generator device serving as a point selective sterilizer

Photochem Photobiol. 2010 Jul-Aug;86(4):890-4. doi: 10.1111/j.1751-1097.2010.00748.x. Epub 2010 May 21.

Abstract

Traditionally, Type II heterogeneous photo-oxidations produce singlet oxygen via external irradiation of a sensitizer and external supply of ground-state oxygen. A potential improvement is reported here. A hollow-core fiber-optic device was developed with an "internal" supply of light and flowing oxygen, and a porous photosensitizer-end capped configuration. Singlet oxygen was delivered through the fiber tip. The singlet oxygen steady-state concentration in the immediate vicinity of the probe tip was ca 20 fm by N-benzoyl-DL-methionine trapping. The device is portable and the singlet oxygen-generating tip is maneuverable, which opened the door to simple disinfectant studies. Complete Escherichia coli inactivation was observed in 2 h when the singlet oxygen sensitizing probe tip was immersed in 0.1 mL aqueous samples of 0.1-4.4 x 10(7) cells. Photobleaching of the probe tip occurred after ca 12 h of use, requiring baking and sensitizer reloading steps for reuse.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / chemistry
  • Escherichia coli / radiation effects
  • Fiber Optic Technology / instrumentation*
  • Fiber Optic Technology / methods
  • Light
  • Oxygen / chemistry
  • Photochemistry
  • Photosensitizing Agents / chemistry*
  • Singlet Oxygen / chemistry*

Substances

  • Photosensitizing Agents
  • Singlet Oxygen
  • Oxygen