Abstract
Cytoplasmic transduction peptide (CTP) is a newly designed transduction peptide, by which special molecules can be carried out and localized into cytoplasmic compartment. Superoxide dismutase (SOD) is a protein that is difficult to go into cytoplasm. In this study, CTP-SOD fusion gene was amplified from human cDNA by PCR, and the active recombinant protein was successfully expressed in Pichia pastoris. HeLa cells pretreated with CTP-SOD showed a significantly improved survival against the pyrogallol-induced oxidative stress, suggesting CTP-SOD could cross the cell membrane more efficiently and protect cells from oxidative stress.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / pharmacology
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Cytoplasm / drug effects
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Cytoplasm / metabolism*
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Genetic Vectors / genetics
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HeLa Cells
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Humans
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism*
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Oxidative Stress / drug effects
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Pichia / genetics
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Pichia / metabolism*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics*
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Recombinant Fusion Proteins / pharmacology
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Signal Transduction / drug effects
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Signal Transduction / physiology
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Superoxide Dismutase / biosynthesis
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Superoxide Dismutase / genetics
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Superoxide Dismutase / metabolism
Substances
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Antioxidants
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Intracellular Signaling Peptides and Proteins
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Recombinant Fusion Proteins
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Superoxide Dismutase