Abstract
This study compares how different DNA extraction methods influence the quantity and quality of DNA yields from stream epilithic biofilms. Interpretations of bacterial community structure, using ARISA, revealed increased variability among samples processed using commercially-available kits, which also yielded lower DNA concentrations than other methods tested.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteria / genetics
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Bacteria / isolation & purification*
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Biofilms*
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DNA Fingerprinting
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DNA, Bacterial / analysis
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DNA, Bacterial / isolation & purification*
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Ecosystem*
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Fresh Water / microbiology
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Polymerase Chain Reaction
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RNA, Ribosomal / analysis
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Rivers / microbiology*
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Sensitivity and Specificity
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Sequence Analysis, DNA
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Water Microbiology*
Substances
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DNA, Bacterial
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RNA, Ribosomal