Two begomovirus isolates (YX2-I and YX2-II) were identified from Clerodendrum cyrtophyllum showing yellow mosaic symptoms collected in Jiangsu province of China. Sequence analysis reveals that YX2-I is a distinct begomovirus species for which the name Clerodendrum golden mosaic Jiangsu virus (ClGMJSV-[CN:YX2:08]) is proposed. YX2-II is an isolate of bipartite begomovirus Clerodendrum golden mosaic China virus (ClGMCNV-[CN:YX2:08]). Infectious clones of the two viruses were constructed and agroinoculated into Nicotiana benthamiana, N. glutinosa, N. tabacum Samsun, Petunia hybrida, Solanum lycopersicum, Capsicum annuum, S. melongena, Glycine max and Gossypium hirsutum plants. ClGMJSV induced leaf curling and stunting symptoms in N. benthamiana, N. glutinosa, N. tabacum Samsun, and P. hybrida, and ClGMCNV infected N. benthamiana, N. glutinosa, and N. tabacum Samsun with severe symptoms and P. hybrida without obvious symptom. Latent infection in N. benthamiana, N. glutinosa, N. tabacum Samsun, and P. hybrida plants was observed when plants were inoculated with ClGMCNV DNA-A alone. In addition, we illustrated that ClGMCNV DNA-A was capable of interacting with the betasatellite associated with Tobacco curly shoot virus (TbCSB) to produce symptoms in N. benthamiana and N. glutinosa plants, and ClGMJSV could interact with TbCSB but not with ClGMCNV DNA-B in N. benthamiana plants.