[To identify a novel HLA-DRB1 allele in Chinese by sequencing]

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Jun;27(3):333-4. doi: 10.3760/cma.j.issn.1003-9406.2010.0.021.
[Article in Chinese]

Abstract

Objective: To identify a novel HLA-DRB1 allele in Chinese.

Methods: A novel HLA-DR allele was detected by PCR-SSP and SBT in a patient with leukemia.

Results: The sequence of the novel allele was different from all other known alleles. The novel allele differed from the closet matching allele HLA-DRB1*1404 by one nucleotide substitution in exon 2, at position 33 T>C, this resulted in an amino acid change from Tyr to His at codon 17.

Conclusion: The novel allele is confirmed as a new HLA allele and it was officially named HLA-DRB1*1461 by WHO Nomenclature Committee in May, 2006.

MeSH terms

  • Alleles*
  • Asian People / genetics
  • HLA-DR Antigens / genetics*
  • HLA-DRB1 Chains
  • Humans
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA

Substances

  • HLA-DR Antigens
  • HLA-DRB1 Chains