Improved molecular detection of Angiostrongylus cantonensis in mollusks and other environmental samples with a species-specific internal transcribed spacer 1-based TaqMan assay

Appl Environ Microbiol. 2010 Aug;76(15):5287-9. doi: 10.1128/AEM.00546-10. Epub 2010 Jun 11.

Abstract

Angiostrongylus cantonensis is the most common cause of human eosinophilic meningitis. Humans become infected by ingesting food items contaminated with third-stage larvae that develop in mollusks. We report the development of a real-time PCR assay for the species-specific identification of A. cantonensis in mollusk tissue.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiostrongylus cantonensis / genetics
  • Angiostrongylus cantonensis / isolation & purification*
  • Animals
  • DNA Primers / genetics
  • DNA, Helminth / chemistry
  • DNA, Helminth / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Environmental Microbiology*
  • Molecular Sequence Data
  • Mollusca / parasitology*
  • Parasitology / methods*
  • Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA

Substances

  • DNA Primers
  • DNA, Helminth
  • DNA, Ribosomal Spacer

Associated data

  • GENBANK/GU587745
  • GENBANK/GU587746
  • GENBANK/GU587747
  • GENBANK/GU587748
  • GENBANK/GU587749
  • GENBANK/GU587750
  • GENBANK/GU587751
  • GENBANK/GU587752
  • GENBANK/GU587753
  • GENBANK/GU587754
  • GENBANK/GU587755
  • GENBANK/GU587756
  • GENBANK/GU587757
  • GENBANK/GU587758
  • GENBANK/GU587759
  • GENBANK/GU587760
  • GENBANK/GU587761
  • GENBANK/GU587762
  • GENBANK/GU733321
  • GENBANK/GU733322
  • GENBANK/GU733323
  • GENBANK/GU733324
  • GENBANK/GU733325