Abstract
We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel. This effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriophage lambda / genetics
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Benzoxazoles / chemistry*
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DNA / chemistry*
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DNA, Viral / chemistry
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Lab-On-A-Chip Devices
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Microscopy, Fluorescence / methods*
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Nanotechnology / instrumentation
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Nanotechnology / methods*
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Quinolinium Compounds / chemistry*
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Silicon Dioxide / chemistry*
Substances
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Benzoxazoles
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DNA, Viral
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Quinolinium Compounds
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1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium
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Silicon Dioxide
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DNA