Enhancement of C2C12 differentiation by perfluorocarbon-mediated oxygen delivery

J Biosci Bioeng. 2010 Sep;110(3):359-62. doi: 10.1016/j.jbiosc.2010.02.012. Epub 2010 Mar 16.

Abstract

We have studied the effect of enhanced oxygen delivery by perfluorocarbons on the differentiation of C2C12 cells. The extent of differentiation was assessed by means of phase contrast/fluorescence microscopy, active tension measurement and the glucose consumption/lactate production rates. We found that enhanced oxygen delivery is suitable for full differentiation of C2C12 cells.

MeSH terms

  • Animals
  • Cell Differentiation
  • Cell Line
  • Fluorocarbons / pharmacokinetics*
  • Mice
  • Muscle Fibers, Skeletal / cytology*
  • Muscle Fibers, Skeletal / metabolism*
  • Myoblasts / cytology*
  • Myoblasts / metabolism*
  • Oxygen / metabolism*

Substances

  • Fluorocarbons
  • Oxygen