Population data and sequence analysis of a 'new' microsatellite locus HumHUU (D16S3433)

Magdalena Konarzewska; Witold Pepinski; A Jagiello; M Spolnicka; I Soltyszewski; A Plucienniczak; J Berent

doi:10.1016/j.fsigen.2010.03.018

Population data and sequence analysis of a 'new' microsatellite locus HumHUU (D16S3433)

Forensic Sci Int Genet. 2010 Oct;4(5):e143-4. doi: 10.1016/j.fsigen.2010.03.018. Epub 2010 Jun 15.

Authors

Magdalena Konarzewska¹, Witold Pepinski, A Jagiello, M Spolnicka, I Soltyszewski, A Plucienniczak, J Berent

Affiliation

¹ Department of Medical Genetics, Warsaw Medical University, Warsaw, Poland.

PMID: 20554491
DOI: 10.1016/j.fsigen.2010.03.018

Abstract

This study reports sequence characteristics and population genetic data on a 'new' STR locus HumHUU (D16S3433) located in the non-coding region of chromosome 16q. Based on a population sample of 306 non-related Polish individuals 205 genotypes and 15 alleles with length range of 157-211bp were distinguished. No deviation from HWE was observed. The sequence analysis of each D16S3433 allele revealed a tetranucleotide repeat motif with a basic sequence structure (AAAA)(0-1)(AAAG)(11-22)(AAAAG)(AAAA)(AG)(AAAAAAG). The power of discrimination is 0.9538, showing a high degree of polymorphism. The presented results demonstrate that the D16S3433 is a useful genetic marker for forensic purposes and paternity testing.

MeSH terms

Alleles
Base Sequence
DNA Primers
Humans
Microsatellite Repeats / genetics*
Polymerase Chain Reaction
Quality Control

Substances

DNA Primers