Erythrocyte binding preference of 16 subtypes of low pathogenic avian influenza and 2009 pandemic influenza A (H1N1) viruses

Vet Microbiol. 2010 Dec 15;146(3-4):346-9. doi: 10.1016/j.vetmic.2010.05.031. Epub 2010 May 31.

Abstract

All 16 subtypes of avian influenza viruses of low pathogenicity (LPAIV) as well as their hemagglutinin (H) antigens, and four 2009 pandemic influenza A (H1N1) virus isolates were assayed for hemagglutinating activity against 5 erythrocyte species: goose, guinea pig, human group O, chicken and horse. Of all viruses and antigens assayed, the highest hemagglutination (HA) titers were obtained with goose and guinea pig erythrocytes. Hemagglutinating activity of replicating LPAIV and LPAIV antigens decreased, in order, with chicken and human group O; meanwhile, horse erythrocytes yielded lowest or no HA titer. Moreover, the 2009 pandemic viruses did not agglutinate both horse and chicken erythrocytes. Our study concluded that goose and guinea pig erythrocytes are the best in HA assay for all subtypes of influenza viruses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chickens
  • Erythrocytes / immunology
  • Erythrocytes / virology*
  • Geese
  • Guinea Pigs
  • Hemagglutination Tests
  • Hemagglutinins / metabolism
  • Horses
  • Humans
  • Influenza A Virus, H1N1 Subtype / immunology*
  • Influenza A Virus, H5N1 Subtype / immunology*
  • Influenza A virus / immunology
  • Influenza A virus / metabolism
  • Influenza in Birds / virology*
  • Influenza, Human / virology*

Substances

  • Hemagglutinins