Abstract
Amongst nalidixic acid-resistant, ciprofloxacin-susceptible Escherichia coli and Klebsiella pneumoniae clinical isolates recovered over a 5-month period from inpatients and outpatients of Attikon University General Hospital (Athens, Greece), only one E. coli was positive for qnrB2 and one K. pneumoniae was positive for qnrA1. Both isolates were extended-spectrum beta-lactamase-negative, metallo-beta-lactamase-positive and carried the bla(VIM-1) gene. Neither of the isolates had mutations in gyrA and parC or carried aac(6')-Ib-cr or qepA. The K. pneumoniae isolate also harboured bla(CMY-13) on the same transferable plasmid with qnrA1. This is the first report of a qnrA1-positive K. pneumoniae and qnrB2-positive E. coli harbouring a concurrent bla(VIM-1) gene.
Copyright (c) 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics
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Conjugation, Genetic
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial*
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Escherichia coli / drug effects*
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Escherichia coli / enzymology
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Escherichia coli / genetics
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Escherichia coli / isolation & purification
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Escherichia coli Infections / microbiology
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Escherichia coli Proteins / genetics
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Greece
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Humans
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Klebsiella Infections / microbiology
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Klebsiella pneumoniae / drug effects*
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Klebsiella pneumoniae / enzymology
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Klebsiella pneumoniae / genetics
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Klebsiella pneumoniae / isolation & purification
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Microbial Sensitivity Tests
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Plasmids*
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Polymerase Chain Reaction
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Quinolones / pharmacology*
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beta-Lactamases / genetics
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA, Bacterial
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Escherichia coli Proteins
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Qnr protein, E coli
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Qnr protein, Klebsiella pneumoniae
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Quinolones
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VIM-1 metallo-beta-lactamase
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beta-Lactamases