False-positive transcription-mediated amplification assay detection of West Nile virus in blood from a patient with viremia caused by an Usutu virus infection

J Clin Microbiol. 2010 Sep;48(9):3338-9. doi: 10.1128/JCM.02501-09. Epub 2010 Jun 30.

Abstract

Detection of West Nile virus (WNV) by nucleic acid amplification technology (NAAT) is used widely to screen blood and organ donations in areas where WNV is endemic. We report a false-positive result of a WNV transcription-mediated amplification assay (TMA) in a patient with viremia that was caused by Usutu virus, a mosquito-borne flavivirus.

MeSH terms

  • Blood / virology
  • Encephalitis Viruses, Japanese / isolation & purification*
  • Encephalitis, Arbovirus / diagnosis*
  • Encephalitis, Arbovirus / virology
  • False Positive Reactions*
  • Flavivirus Infections / diagnosis*
  • Flavivirus Infections / virology
  • Humans
  • Nucleic Acid Amplification Techniques / methods*
  • RNA, Viral / blood
  • Viremia
  • Virology / methods*
  • West Nile Fever / diagnosis
  • West Nile Fever / virology
  • West Nile virus / isolation & purification*

Substances

  • RNA, Viral