Merlin expression in pediatric anaplastic ependymomas real time PCR study

Fetal Pediatr Pathol. 2010;29(4):245-54. doi: 10.3109/15513811003789644.

Abstract

The most common genetic abnormalities of ependymomas involve the chromosome 22 where there is the oncosuppressor gene neurofibromin 2 (NF2). NF2 mutations are primarily encountered in spinal lesions. In contrast, NF2 alterations do not seem related to tumor grade. We studied the NF2 expression through a real-time polymerase chain reaction in 25 pediatric anaplastic ependymomas. We compared the NF2 expression in neoplastic and non-neoplastic tissues, in supratentorial and infratentorial ependymomas and in primitive and non-primitive tumors (recurrences and metastases). Statistical analysis did not prove significant differences. Our results suggest that NF2 alterations are not typical of intracranial anaplastic ependymomas.

Publication types

  • Case Reports

MeSH terms

  • Adolescent
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / pathology
  • Brain Neoplasms / surgery
  • Child
  • Child, Preschool
  • DNA, Neoplasm / analysis
  • Ependymoma / genetics*
  • Ependymoma / secondary
  • Ependymoma / surgery
  • Female
  • Gene Expression
  • Humans
  • Infant
  • Infratentorial Neoplasms / genetics
  • Infratentorial Neoplasms / pathology
  • Infratentorial Neoplasms / surgery
  • Male
  • Neoplasm Metastasis
  • Neoplasm Recurrence, Local / genetics
  • Neoplasm Recurrence, Local / pathology
  • Neurofibromin 2 / genetics*
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Supratentorial Neoplasms / genetics
  • Supratentorial Neoplasms / pathology
  • Supratentorial Neoplasms / surgery

Substances

  • DNA, Neoplasm
  • Neurofibromin 2
  • RNA, Messenger