Abstract
We report the imaging of hepatocellular carcinoma cells and the immunoassay for alpha fetoprotein (AFP) using CdTe/CdS/ZnS core-shell-shell QDs. Stable and high PLQY (20%-48%) CdTe/CdS/ZnS core-shell-shell QDs were synthesized by a stepwise process. Bioconjugation of the core-shell-shell QDs with streptavidin (SA) was successfully applied in immunofluorescent imaging of the human hepatocellular carcinoma (HCC) cell line HepG2.2.15. Furthermore, the thioglycolic acid (TGA)-capped CdTe/CdS/ZnS core-shell-shell QDs fluorescence lifetime is longer than fluorescein, so it was first engaged to conjugate with antigen for the determination of protein (AFP) by fluorescence polarization immunoassay.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cadmium Compounds / chemical synthesis*
-
Carcinoma, Hepatocellular / diagnosis*
-
Carcinoma, Hepatocellular / pathology
-
Cell Line, Tumor
-
Chromatography, High Pressure Liquid
-
Diagnostic Imaging / methods*
-
Fluorescein-5-isothiocyanate / metabolism
-
Humans
-
Immunoassay
-
Liver Neoplasms / diagnosis
-
Liver Neoplasms / pathology
-
Microscopy, Fluorescence
-
Quantum Dots*
-
Spectrophotometry, Ultraviolet
-
Sulfides / chemical synthesis*
-
Tellurium
-
Zinc Compounds / chemical synthesis*
-
alpha-Fetoproteins / analysis*
-
alpha-Fetoproteins / immunology
Substances
-
Cadmium Compounds
-
Sulfides
-
Zinc Compounds
-
alpha-Fetoproteins
-
cadmium sulfide
-
Fluorescein-5-isothiocyanate
-
zinc sulfide
-
Tellurium
-
cadmium telluride