miR-126 functions as a tumour suppressor in human gastric cancer

Cancer Lett. 2010 Dec 1;298(1):50-63. doi: 10.1016/j.canlet.2010.06.004. Epub 2010 Jul 8.

Abstract

MicroRNAs have emerged as important gene regulators and are recognised as key players in carcinogenesis. In the present study, we show that miR-126 was significantly down-regulated in gastric cancer tissues compared with matched normal tissues and was associated with clinicopathological features, including tumour size, lymph node metastasis, local invasion and tumour-node-metastasis (TNM) stage. Ectopic expression of miR-126 in SGC-7901 gastric cancer cells potently inhibited cell growth by inducing cell cycle arrest in G0/G1 phase, migration and invasion in vitro as well as tumorigenicity and metastasis in vivo. Mechanistically, we identified the adaptor protein Crk as a target of miR-126. Taken together, our results suggest that miR-126 may function as a tumour suppressor in gastric cancer, with Crk as a direct target.

MeSH terms

  • Animals
  • Cell Cycle / genetics
  • Cell Growth Processes / genetics
  • Cell Line, Tumor
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic
  • Genes, Tumor Suppressor*
  • Humans
  • Male
  • Mice
  • Mice, Nude
  • MicroRNAs / biosynthesis
  • MicroRNAs / genetics*
  • Proto-Oncogene Proteins c-crk / biosynthesis
  • Proto-Oncogene Proteins c-crk / genetics
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology
  • Transfection

Substances

  • CRK protein, human
  • MIRN126 microRNA, human
  • MicroRNAs
  • Proto-Oncogene Proteins c-crk