Evaluation of human osteosarcoma cell line genotoxicity effects of mineral trixoide aggregate and calcium silicate cements

J Endod. 2010 Jul;36(7):1158-62. doi: 10.1016/j.joen.2010.03.039. Epub 2010 May 23.

Abstract

Introduction: Mineral trioxide aggregate (MTA) and calcium silicate (CS) cements exhibit acceptable levels of cytocompatibility. The aim of the present study was to evaluate MTA and CS cement genotoxicity in a human osteosarcoma cell line (MG63).

Methods: A mitochondrial colorimetric assay was used to evaluate the MG63 survival rate. A DNA precipitation assay was used to detect the MG63 DNA damage after contact with MTA or CS cement extracts.

Results: The results showed that MTA and CS are cytocompatible with MG63 cells. There was no significant difference in the survival rate with MTA and CS materials (P > .05). Neither MTA nor CS cements causing MG63 cell DNA damage showed significant genotoxicity (P > .05).

Conclusions: Both MTA and CS cements are compatible with MG63 cells, and they are not cancer-causing agents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum Compounds / toxicity*
  • Aluminum Oxide / analysis
  • Biocompatible Materials / toxicity
  • Calcium Compounds / analysis
  • Calcium Compounds / toxicity*
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Colorimetry
  • DNA / analysis
  • DNA Damage
  • Drug Combinations
  • Ferric Compounds / analysis
  • Humans
  • Magnesium Oxide / analysis
  • Mutagens / toxicity*
  • Osteosarcoma / pathology*
  • Oxides / analysis
  • Oxides / toxicity*
  • Root Canal Filling Materials / toxicity*
  • Silicates / analysis
  • Silicates / toxicity*
  • Silicon Dioxide / analysis
  • Zinc Oxide / analysis

Substances

  • Aluminum Compounds
  • Biocompatible Materials
  • Calcium Compounds
  • Drug Combinations
  • Ferric Compounds
  • Mutagens
  • Oxides
  • Root Canal Filling Materials
  • Silicates
  • mineral trioxide aggregate
  • ferric oxide
  • Magnesium Oxide
  • Silicon Dioxide
  • DNA
  • lime
  • Aluminum Oxide
  • calcium silicate
  • Zinc Oxide