Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

Steven Y C Tong; Rachael A Lilliebridge; Deborah C Holt; Geoffrey W Coombs; Bart J Currie; Philip M Giffard

doi:10.1016/j.diagmicrobio.2010.03.015

Rapid detection of H and R Panton-Valentine leukocidin isoforms in Staphylococcus aureus by high-resolution melting analysis

Diagn Microbiol Infect Dis. 2010 Aug;67(4):399-401. doi: 10.1016/j.diagmicrobio.2010.03.015.

Authors

Steven Y C Tong¹, Rachael A Lilliebridge, Deborah C Holt, Geoffrey W Coombs, Bart J Currie, Philip M Giffard

Affiliation

¹ Menzies School of Health Research, Darwin, PO Box 41096 Casuarina, NT 0811, Australia. [email protected]

PMID: 20638613
DOI: 10.1016/j.diagmicrobio.2010.03.015

Abstract

We designed a single-step, closed-tube, real-time polymerase chain reaction and high-resolution melting assay to simultaneously detect the presence of the Panton-Valentine leukocidin gene and discriminate histidine and arginine isoforms. Of 223 Staphylococcus aureus isolates from northern Australia, isoforms clustered by clonal complex (CC). All CC93 isolates harbored the arginine isoform.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Australia
Bacterial Proteins / genetics*
Bacterial Toxins / genetics*
Exotoxins / genetics*
Humans
Leukocidins / genetics*
Polymerase Chain Reaction / methods
Protein Isoforms / genetics
Staphylococcal Infections / microbiology
Staphylococcus aureus / genetics*
Staphylococcus aureus / isolation & purification
Transition Temperature*

Substances

Bacterial Proteins
Bacterial Toxins
Exotoxins
Leukocidins
Panton-Valentine leukocidin
Protein Isoforms