Abstract
MarR is a key regulator of the marRAB operon involved in antibiotic resistance and solvent stress tolerance in Escherichia coli. We show that two metabolic intermediates, 2,3-dihydroxybenzoate and anthranilate, involved in enterobactin and tryptophan biosynthesis, respectively, can activate marRAB transcription. We also found that a third intermediate involved in ubiquinone biosynthesis, 4-hydroxybenzoate, activates marRAB transcription in the absence of TolC. Of the three, however, only 2,3-dihydroxybenzoate directly binds MarR and affects its activity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Proteins / genetics*
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DNA-Binding Proteins / genetics*
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Escherichia coli / drug effects*
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins / genetics*
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Gene Expression Regulation, Bacterial
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Hydroxybenzoates / pharmacology
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Membrane Transport Proteins / genetics
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Operon / drug effects*
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Operon / genetics
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Parabens / pharmacology
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Promoter Regions, Genetic / drug effects
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Promoter Regions, Genetic / genetics
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Protein Binding / drug effects
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Protein Binding / genetics
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Repressor Proteins / genetics*
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ortho-Aminobenzoates / pharmacology
Substances
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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DNA-Binding Proteins
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Escherichia coli Proteins
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Hydroxybenzoates
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MarA protein, E coli
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MarR protein, E coli
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Membrane Transport Proteins
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Parabens
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Repressor Proteins
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multiple antibiotic resistance protein B
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ortho-Aminobenzoates
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tolC protein, E coli
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anthranilic acid
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2,3-dihydroxybenzoic acid
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4-hydroxybenzoic acid