Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR

Benedetta Peruzzi; Martina Serra; Chiara Pescucci; Michela Sica; Sonia Lastraioli; Tommaso Rondelli; Simona Pedemonte; Antonio Maria Risitano; Maria De Angioletti; Patrizia Piccioli; Rosario Notaro

doi:10.1016/j.mcp.2010.07.002

Easy genotyping of complement C3 'slow' and 'fast' allotypes by tetra-primer amplification refractory mutation system PCR

Mol Cell Probes. 2010 Dec;24(6):401-2. doi: 10.1016/j.mcp.2010.07.002. Epub 2010 Jul 22.

Authors

Benedetta Peruzzi¹, Martina Serra, Chiara Pescucci, Michela Sica, Sonia Lastraioli, Tommaso Rondelli, Simona Pedemonte, Antonio Maria Risitano, Maria De Angioletti, Patrizia Piccioli, Rosario Notaro

Affiliation

¹ Laboratory of Genetics and Gene Transfer, Core Research Laboratory, Istituto Toscano Tumori (ITT), AOU Careggi, Firenze, Italy.

PMID: 20655380
DOI: 10.1016/j.mcp.2010.07.002

Abstract

Complement C3 'slow' and 'fast' allotypes are associated with immune-mediated disorders and may affect the outcome of renal transplantation. We report a tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) that provides a rapid, reproducible and cost-effective method to genotype both complement C3 'slow' and 'fast' alleles by a single tube reaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles*
Cell Line
Complement C3 / genetics*
DNA Primers / metabolism*
Genotype
Humans
Mutation / genetics*
Polymerase Chain Reaction / methods*
Polymorphism, Single Nucleotide / genetics

Substances

Complement C3
DNA Primers