Superparamagnetic nanocrystal clusters for enrichment of low-abundance peptides and proteins

Chem Commun (Camb). 2010 Sep 7;46(33):6174-6. doi: 10.1039/c0cc01734e. Epub 2010 Jul 23.

Abstract

Clusters of densely packed hydrophobic superparamagnetic gamma-Fe(2)O(3) nanocrystals have been developed as a powerful adsorbent for enrichment of peptides and proteins, with advantages such as high enrichment efficiency, fast processing, easy magnetic separation, and direct mass analysis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Angiotensin II / isolation & purification
  • Cytochromes c / isolation & purification
  • Ferric Compounds / chemistry
  • Hydrophobic and Hydrophilic Interactions
  • Magnetics*
  • Microscopy, Electron, Transmission
  • Nanoparticles / chemistry*
  • Peptides / isolation & purification*
  • Proteins / isolation & purification*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Surface Properties

Substances

  • Ferric Compounds
  • Peptides
  • Proteins
  • Angiotensin II
  • ferric oxide
  • Cytochromes c