Human motor neuron generation from embryonic stem cells and induced pluripotent stem cells

Cell Mol Life Sci. 2010 Nov;67(22):3837-47. doi: 10.1007/s00018-010-0463-y. Epub 2010 Jul 29.

Abstract

Motor neuron diseases (MNDs) are a group of neurological disorders that selectively affect motor neurons. There are currently no cures or efficacious treatments for these diseases. In recent years, significant developments in stem cell research have been applied to MNDs, particularly regarding neuroprotection and cell replacement. However, a consistent source of motor neurons for cell replacement is required. Human embryonic stem cells (hESCs) could provide an inexhaustible supply of differentiated cell types, including motor neurons that could be used for MND therapies. Recently, it has been demonstrated that induced pluripotent stem (iPS) cells may serve as an alternative source of motor neurons, since they share ES characteristics, self-renewal, and the potential to differentiate into any somatic cell type. In this review, we discuss several reproducible methods by which hESCs or iPS cells are efficiently isolated and differentiated into functional motor neurons, and possible clinical applications.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cell Separation / methods
  • Cell- and Tissue-Based Therapy
  • Embryonic Stem Cells / cytology*
  • Embryonic Stem Cells / metabolism
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Motor Neuron Disease / metabolism
  • Motor Neuron Disease / therapy
  • Motor Neurons / cytology*
  • Motor Neurons / metabolism
  • Motor Neurons / transplantation
  • Neurogenesis*