Background and objective: As a barrier to metastases, cells normally undergo apoptosis after they lose contact with their extra cellular matrix (ECM). This process has been termed "anoikis". Tumour cells that acquire malignant potential have developed mechanisms to resist anoikis and thereby survive after detachment from their primary site while traveling through the lymphatic and circulatory systems. This "anoikis resistance" is considered the first step to tumor metastases. The aim of this study was to screen metastasis-associated genes from anoikis resistant and adherent growth A549 lung cancer cell by Human Genome Array.
Methods: Establish anoikis resistant A549 lung cancer cell lines by using poly-hydroxyethyl methacrylate resin processed petri dishes, which causes cell free from adherent. The different expressed gene between anoikis resistant A549 cell and adherent growth A549 cell was tested using human V2.0 whole-genome oligonucleotide microarray, a product of Capitalbio Corporation, Beijing. Screen metastasis-associated genes.
Results: 745 different expressed genes were screened, including 63 highly metastasis-associated genes.
Conclusion: The successfully established anoikis resistant A549 cell lines and screened different expressed genes provide us basis for further research on metastasis of lung cancer.
背景与目的: 正常上皮或内皮细胞脱离细胞外基质会发生脱落凋亡，但肿瘤细胞可不依赖细胞基质生长而不发生凋亡，这一现象被称为抗脱落凋亡，目前国内外相关研究均认为抗脱落凋亡是肿瘤发生转移的始动环节。本实验旨在比较抗脱落凋亡及正常贴壁生长肺腺癌A549细胞基因组表达差异，从中筛选肺癌转移相关基因。
方法: 利用多聚羟乙基甲基丙烯酸树脂处理培养皿，致细胞无法贴壁生长，建立抗脱落凋亡肺癌A549细胞系；利用北京博奥生物芯片公司的人类V2.0全基因组寡核苷酸微阵列芯片，检测其与正常贴壁生长A549细胞的基因表达差异性，筛选肺癌转移相关基因。
结果: 共得到表达差异的基因745个，从中筛选出63个与肺癌转移密切相关的基因。
结论: 成功建立抗脱落凋亡肺癌A549细胞系并筛选出转移相关差异表达基因，为进一步研究肺癌转移相关信号转导通路及其它相关研究提供依据。