Background and objective: The actual evaluation of immunological function is significant for studing the tumor development and devising a treatment in time. The aim of this study is to evaluate the immunological function of advanced lung cancer patients systematically, and to discuss the clinical significance.
Methods: The nucleated cell amounts of advanced lung cancer patients and the healthy individuals were counted. The immune cell subsets and the levels of IL-4, INF-gamma, perforin and granzyme in CD8+T cells by the flow cytometry were measured. The proliferation activity and the inhibition ratio of immune cells to several tumor cell lines were evaluated by MTT assay.
Results: The absolute amounts and subsets of T, B, NK cells of advanced lung cancer patients were lower than the healthy individuals (P < 0.05); However, the proportion of regulatory T cells of advanced lung cancer patients (4.00 +/- 1.84)% was lower than the healthy individuals (1.27 +/- 0.78)% (P < 0.05). The positive rates of IFN-gamma perforin, granzyme in CD8+T cells decreased while them in IL-4 did not in the advanced lung cancer patients compared to the healthy control group (P < 0.05). The proliferation activity of immune cells, the positive rate of PPD masculine and the inhibition ratio to tumor cells in the advanced lung cancer patients was lower than the healthy subsets obviously (P < 0.05).
Conclusion: There was a significant immune depression in the advanced lung cancer patients compared to the healthy individuals.
背景与目的: 真实评价免疫功能对明确肿瘤的发生发展及给予适时治疗具有重要意义。本研究旨在系统评价晚期肺癌患者免疫功能及意义。
方法: 计数晚期肺癌患者和健康人外周血免疫细胞数；用流式细胞仪测定免疫细胞亚群比例和细胞内IL-4、INF-γ及穿孔素、颗粒酶水平；用MTT法评价淋巴细胞对肿瘤细胞株的抑制率及其增殖活性。
结果: 晚期肺癌患者组T、B、NK细胞绝对数及亚群比例均显著低于健康组（P < 0.05）；但患者组的调节性T细胞（4.00±1.84）%明显高于健康组（1.27±0.78）%（P < 0.05）。患者组的CD8+T细胞中IFN-γ、穿孔素及颗粒酶阳性率均显著低于健康组（P < 0.05）；而IL-4正好相反。患者组的免疫细胞增殖能力、PPD阳性率及对瘤细胞株的抑制率显著低于健康组（P < 0.05）。
结论: 晚期肺癌患者免疫功能较健康人明显低下。