Retinoic acid and alpha-difluoromethylornithine induce different expression of neural-specific cell adhesion molecules in differentiating neuroblastoma cells

Prog Clin Biol Res. 1991:366:283-91.

Abstract

Human neuroblastoma cells SK-N-BE(2) can be induced to differentiate towards a neuronal phenotype by retinoic acid (RA) or a schwannian/glial phenotype by alpha-difluoromethylornithine (DFMO), producing differential binding of 14 antibodies (MAbs). RA induced the expression of the neural cell adhesion molecule, NCAM (also confirmed by northern blot); whereas DFMO enhanced the binding of MAbs UJ181.4, UJ127.11 which recognise an identical protein doublet of 220-240 kDa, thought to be the L1 protein(s). The data presented demonstrate that neuroblastoma cells differentiate toward separate phenotypes associated with a specific induction of two different adhesion molecules, NCAM on neuronal cells and L1 on schwannian/glial cells.

MeSH terms

  • Antibodies, Monoclonal
  • Blotting, Northern
  • Blotting, Western
  • Cell Adhesion Molecules, Neuronal / analysis
  • Cell Adhesion Molecules, Neuronal / genetics*
  • Cell Differentiation* / drug effects
  • Cell Line
  • Eflornithine / pharmacology*
  • Fluorescent Antibody Technique
  • Gene Expression / drug effects
  • Humans
  • Models, Neurological
  • Molecular Weight
  • Neuroblastoma / genetics
  • Neuroblastoma / pathology
  • Neurons / cytology
  • Phenotype
  • Tretinoin / pharmacology*

Substances

  • Antibodies, Monoclonal
  • Cell Adhesion Molecules, Neuronal
  • Tretinoin
  • Eflornithine