Abstract
CFP21 is a major secreted protein of Mycobacterium tuberculosis (Mtb) which is considered as a promising antigen for immunotherapy. To identify CFP21-derived HLA-A*0201 restricted epitopes, a series of native peptides and their analogues were predicted with prediction programs and synthesized. The native peptide, p134 (AVADHVAAV), and its analogues, p134-1Y2L and p134-1Y2L9L, showed potent binding affinity and stability to HLA-A*0201 molecule. In ELISPOT assay, the cytotoxic T lymphocytes (CTLs) induced by these peptides could release IFN-γ. In cytotoxicity assay, the CTLs induced by p134 and p134-1Y2L9L could specifically lyse peptide-loaded T2 cells. In these two assays, the native peptide, p134, showed the most potent activity. Our results indicated that p134 could be a novel epitope which could serve as a good candidate to develop peptide vaccines against M. tuberculosis.
Copyright © 2010 Elsevier B.V. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antigens, Bacterial / chemistry
-
Antigens, Bacterial / immunology
-
Antigens, Bacterial / metabolism*
-
Cells, Cultured
-
Cytotoxicity, Immunologic
-
Epitope Mapping
-
Epitopes, T-Lymphocyte / chemistry
-
Epitopes, T-Lymphocyte / immunology
-
Epitopes, T-Lymphocyte / metabolism*
-
HLA-A Antigens / metabolism
-
HLA-A2 Antigen
-
Humans
-
Interferon-gamma / metabolism
-
Lymphocyte Activation
-
Mycobacterium tuberculosis / immunology*
-
Peptide Fragments / chemical synthesis
-
Peptide Fragments / immunology
-
Peptide Fragments / metabolism*
-
Protein Binding
-
T-Lymphocytes, Cytotoxic / cytology
-
T-Lymphocytes, Cytotoxic / immunology
-
T-Lymphocytes, Cytotoxic / metabolism*
-
Tuberculosis / immunology
-
Tuberculosis / therapy*
-
Vaccines, Subunit*
Substances
-
Antigens, Bacterial
-
CFP21 protein, Mycobacterium tuberculosis
-
Epitopes, T-Lymphocyte
-
HLA-A Antigens
-
HLA-A*02:01 antigen
-
HLA-A2 Antigen
-
Peptide Fragments
-
Vaccines, Subunit
-
Interferon-gamma