Secretory phospholipase A2: a marker of infection in febrile children presenting to a pediatric ED

Am J Emerg Med. 2011 Nov;29(9):1163-8. doi: 10.1016/j.ajem.2010.06.024. Epub 2010 Aug 13.

Abstract

Background: Fever is a common presenting complaint to the emergency department (ED), and the evaluation of the febrile child remains a challenging task.

Objective: The aim of this study was to examine the relationship between secretory phospholipase A2 (sPLA2) and infection in febrile children.

Methods: A prospective convenience sample of children presenting with fever to an urban pediatric ED were studied. Blood and urine cultures, a complete blood count, and serum concentrations of sPLA2 were obtained, and patients were compared based on their final diagnosis of either a viral or bacterial infection.

Results: In the 76 patients enrolled, 60 were diagnosed with a viral infection, 14 with a bacterial infection, 1 with Kawasaki disease, and 1 with acute lymphoblastic leukemia. The difference in the serum concentration of sPLA2 in patients with viral infections (22 ± 34 ng/mL) versus those with bacterial infections (190 ± 179 ng/mL) was statistically significant (P < .0001). Receiver operator characteristic curve analysis revealed that sPLA2 was more accurate at predicting bacterial infection (area under the curve = 0.89) than the total white blood cell count (area under the curve = 0.71) and that a value of more than 20 ng/mL had a sensitivity of 93%, specificity of 67%, positive predictive value of 39%, and negative predictive value of 97%.

Conclusion: Secretory phospholipase A2 differs significantly in children with viral versus bacterial infection and seems to be a reliable screening test for bacterial infection in febrile children.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adolescent
  • Bacterial Infections / blood
  • Bacterial Infections / diagnosis
  • Biomarkers / blood
  • Child
  • Child, Preschool
  • Emergency Service, Hospital*
  • Female
  • Fever / blood*
  • Fever / microbiology
  • Humans
  • Infant
  • Infant, Newborn
  • Infections / blood*
  • Infections / diagnosis
  • Leukocyte Count
  • Male
  • Mucocutaneous Lymph Node Syndrome / blood
  • Mucocutaneous Lymph Node Syndrome / diagnosis
  • Phospholipases A2, Secretory / blood*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / blood
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / diagnosis
  • Predictive Value of Tests
  • Prospective Studies
  • ROC Curve
  • Sensitivity and Specificity
  • Statistics, Nonparametric
  • Virus Diseases / blood
  • Virus Diseases / diagnosis

Substances

  • Biomarkers
  • Phospholipases A2, Secretory