The effect of obesity on testicular function by insulin-like factor 3, inhibin B, and leptin concentrations in obese adolescents according to pubertal stages

Clin Biochem. 2010 Oct;43(15):1236-40. doi: 10.1016/j.clinbiochem.2010.07.026. Epub 2010 Aug 14.

Abstract

Objectives: The aim of the present study is to investigate the effect of obesity on testicular function by evaluating reproductive hormones, inhibinB, insulin-like 3(INSL3), and leptin, in obese and non-obese adolescents according to pubertal Tanner stages.

Design and methods: Eighty adolescent boys were grouped (n=20) as; Group1: obese-Tanner2, Group2: non-obese-Tanner2, Group3: obese-Tanner4, Group4: non-obese-Tanner4. Serum INSL3, luteinizing hormone, follicle-stimulating hormone, total testosterone, free testosterone, estradiol, sex hormone binding globulin, inhibin B and leptin levels were assessed in all groups.

Results: INSL3 levels were significantly lower in obese adolescents compared to non-obese boys (p=0.003, Tanner2) and (p=0.031, Tanner4). There was a negative correlation between INSL3 and leptin (r=-0.468, p=0.001). The negative correlation between INSL3 and BMISDS indicates that pubertal obesity leads to Leydig cell impairment.

Conclusions: This study demonstrated for the first time in the literature that obesity effects testicular Leydig cell function starting from Tanner stage 2.

MeSH terms

  • Adolescent
  • Body Mass Index
  • Case-Control Studies
  • Child
  • Humans
  • Inhibins / blood*
  • Insulin / blood*
  • Leptin / blood*
  • Luteinizing Hormone / blood
  • Male
  • Obesity / blood*
  • Obesity / physiopathology*
  • Proteins
  • Puberty / blood*
  • Testis / physiopathology*
  • Testosterone / blood

Substances

  • Insulin
  • Leptin
  • Leydig insulin-like protein
  • Proteins
  • inhibin B
  • Testosterone
  • Inhibins
  • Luteinizing Hormone