Expression of micro-RNA-145 is regulated by a highly conserved genomic sequence 3' to the pre-miR

J Cell Physiol. 2011 Mar;226(3):602-7. doi: 10.1002/jcp.22368.

Abstract

Micro-RNA-145 (miR145), a tumor suppressor miR, dramatically inhibits growth of cancer cells in culture and plays a significant role in human stem cells differentiation. We have isolated a human genomic sequence of 864 bp comprising the pre-miR and its flanking sequences. The cloned miR145 genomic sequence expresses a mature miR145 in transfected cells. We show here that flanking sequences on either side of the pre-miR sequence can modulate its expression levels. Surprisingly, a highly conserved sequence 3' to the pre-miR plays a crucial role in miR145 expression.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • 3' Untranslated Regions / genetics*
  • Base Sequence
  • Blotting, Northern
  • Cell Line, Tumor
  • Clone Cells
  • Conserved Sequence / genetics*
  • Gene Expression Regulation, Neoplastic*
  • Genome, Human / genetics*
  • Humans
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Molecular Sequence Data
  • Mutation / genetics
  • RNA Precursors / genetics*
  • RNA Precursors / metabolism
  • Transfection

Substances

  • 3' Untranslated Regions
  • MIRN145 microRNA, human
  • MicroRNAs
  • RNA Precursors