Human rhinovirus C infections mirror those of human rhinovirus A in children with community-acquired pneumonia

J Clin Virol. 2010 Oct;49(2):94-9. doi: 10.1016/j.jcv.2010.07.013. Epub 2010 Aug 21.

Abstract

Background: Human rhinoviruses (HRVs) are among the most common causes of community-acquired pneumonia (CAP) in children. However, the differential roles of the three HRV species HRV-A, HRV-B, and HRV-C in pediatric CAP are not fully understood.

Objective: To determine the distribution of HRV species and their roles in children hospitalized with CAP in Beijing, China.

Study design: Nasopharyngeal aspirates were collected between April 2007 and March 2008 from 554 children with a primary diagnosis of CAP. HRVs in the clinical samples were detected by RT-PCR and by sequencing. Infections with other respiratory viruses were identified by PCR.

Results: HRVs were detected in 99 patients (17.87%). Among these patients, 51.52% tested positive for HRV-A, 38.38% for HRV-C, and 10.10% for HRV-B. HRVs were detected throughout the study period. The monthly distribution of HRV infections varied with HRV species. Median age, gender, symptoms, severity, and duration of hospitalization for single HRV-C infections were similar to those observed for single HRV-A infections. Co-infections with other respiratory viruses were detected in 57.58% of the HRV-positive children. HRV/RSV dual infections were correlated with a higher frequency of shortness of breath (HRV-A group, P(2 tail)= 0.01; HRV-C group, P(2 tail) = 0.015) and lower median ages (HRV-A group, P(2 tail) = 0.049; HRV-C group, P(2 tail) = 0.009).

Conclusion: Our study shows that HRV-C strains circulate at a prevalence intermediate between HRV-A and HRV-B. The severity of clinical manifestations for HRV-C is comparable to that for HRV-A in children with CAP. These findings point to an important role of both HRV-A and HRV-C in pediatric CAP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Child
  • Child, Preschool
  • China / epidemiology
  • Community-Acquired Infections / epidemiology*
  • Community-Acquired Infections / pathology
  • Community-Acquired Infections / virology*
  • Female
  • Hospitalization / statistics & numerical data
  • Humans
  • Infant
  • Male
  • Molecular Sequence Data
  • Nasopharynx / virology
  • Picornaviridae Infections / epidemiology*
  • Picornaviridae Infections / pathology
  • Picornaviridae Infections / virology*
  • Pneumonia, Viral / epidemiology*
  • Pneumonia, Viral / pathology
  • Pneumonia, Viral / virology*
  • Prevalence
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Rhinovirus / classification
  • Rhinovirus / genetics
  • Rhinovirus / isolation & purification*
  • Sequence Analysis, DNA

Substances

  • RNA, Viral

Associated data

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