Abstract
We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Caenorhabditis / classification
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Caenorhabditis / drug effects*
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Caenorhabditis / genetics*
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Drug Resistance / drug effects
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Drug Resistance / genetics*
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Genetic Markers / genetics
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Genetic Vectors / genetics
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Gentamicins / pharmacology*
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Neomycin / pharmacology
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Selection, Genetic / drug effects*
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Selection, Genetic / genetics*
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Transgenes / genetics*
Substances
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Genetic Markers
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Gentamicins
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antibiotic G 418
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Neomycin