Synthesis of K48-linked diubiquitin using dual native chemical ligation at lysine

Chem Commun (Camb). 2010 Oct 14;46(38):7199-201. doi: 10.1039/c0cc01382j. Epub 2010 Aug 25.

Abstract

The dual native chemical ligation at lysine strategy was revised by replacing the acid-labile Cbz protecting group with photolabile NVOC at the 4-mercaptolysine side chain. The optimized strategy was subsequently applied to the synthesis of K48-linked diubiquitin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzaldehydes / chemical synthesis
  • Benzaldehydes / chemistry
  • Lysine / chemical synthesis
  • Lysine / chemistry*
  • Ubiquitins / chemical synthesis*
  • Ubiquitins / chemistry

Substances

  • Benzaldehydes
  • NVOC-chloride
  • Ubiquitins
  • Lysine