Comprehensive embryo analysis of advanced maternal age-related aneuploidies and mosaicism by short comparative genomic hybridization

Mariona Rius; Gemma Daina; Albert Obradors; Laia Ramos; Esther Velilla; Sílvia Fernández; Olga Martínez-Passarell; Jordi Benet; Joaquima Navarro

doi:10.1016/j.fertnstert.2010.07.1051

Comprehensive embryo analysis of advanced maternal age-related aneuploidies and mosaicism by short comparative genomic hybridization

Fertil Steril. 2011 Jan;95(1):413-6. doi: 10.1016/j.fertnstert.2010.07.1051. Epub 2010 Aug 24.

Authors

Mariona Rius¹, Gemma Daina, Albert Obradors, Laia Ramos, Esther Velilla, Sílvia Fernández, Olga Martínez-Passarell, Jordi Benet, Joaquima Navarro

Affiliation

¹ Unitat de Biologia Cellular i Genètica Mèdica, Facultat de Medicina, Universitat Autònoma de Barcelona, Bellaterra, Spain. [email protected]

PMID: 20797709
DOI: 10.1016/j.fertnstert.2010.07.1051

Abstract

The short comparative genomic hybridization (short-CGH) method was used to perform a comprehensive cytogenetic study of isolated blastomeres from advanced maternal age embryos, discarded after fluorescent in situ hybridization (FISH) preimplantation genetic screening (PGS), detecting aneuploidies (38.5% of which corresponded to chromosomes not screened by 9-chromosome FISH), structural aberrations (31.8%), and mosaicism (77.3%). The short-CGH method was subsequently applied in one PGS, achieving a twin pregnancy.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Abortion, Habitual / diagnosis
Adult
Aneuploidy*
Blastocyst / physiology
Chromosome Aberrations
Comparative Genomic Hybridization / methods*
Female
Genetic Testing / methods
Humans
In Situ Hybridization, Fluorescence
Maternal Age*
Mosaicism*
Pregnancy
Pregnancy Outcome
Preimplantation Diagnosis / methods*