Melt processing of chitosan-based fibers and fiber-mesh scaffolds for the engineering of connective tissues

Macromol Biosci. 2010 Dec 8;10(12):1495-504. doi: 10.1002/mabi.201000011. Epub 2010 Aug 26.

Abstract

We report the production of chitosan-based fibers and chitosan fiber-mesh structures by melt processing (solvent-free) to be used as tissue-engineering scaffolds. The melt-based approach used to produce the scaffolds does not change their main characteristics, including the surface roughness and microporosity. The porosity, pore size, interconnectivity and mechanical performance of the scaffolds are all within the range required for various tissue-engineering applications. Biological assessments are performed in direct-contact assays. Cells are able to colonize the scaffold, including the inner porous structure. The cells show high indices of viability in all of the scaffold types.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Butylene Glycols
  • Cell Adhesion / physiology*
  • Cell Line, Tumor
  • Cell Survival
  • Chitosan / chemistry*
  • Connective Tissue Cells / cytology*
  • Freezing
  • Humans
  • Materials Testing
  • Microscopy, Electron, Scanning
  • Polyesters
  • Polymers
  • Porosity
  • Tetrazolium Salts
  • Thiazoles
  • Tissue Engineering / methods*
  • Tissue Scaffolds*
  • X-Ray Microtomography

Substances

  • Butylene Glycols
  • Polyesters
  • Polymers
  • Tetrazolium Salts
  • Thiazoles
  • bionole
  • poly(butylene adipate-co-butylene terephthalate)
  • Chitosan
  • thiazolyl blue