Mass spectrometry in high-throughput proteomics: ready for the big time

Tommy Nilsson; Matthias Mann; Ruedi Aebersold; John R Yates 3rd; Amos Bairoch; John J M Bergeron

doi:10.1038/nmeth0910-681

Mass spectrometry in high-throughput proteomics: ready for the big time

Nat Methods. 2010 Sep;7(9):681-5. doi: 10.1038/nmeth0910-681.

Authors

Tommy Nilsson¹, Matthias Mann, Ruedi Aebersold, John R Yates 3rd, Amos Bairoch, John J M Bergeron

Affiliation

¹ Research Institute of the McGill University Health Centre, McGill University, Montreal, Quebec, Canada. [email protected]

PMID: 20805795
DOI: 10.1038/nmeth0910-681

Abstract

Mass spectrometry has evolved and matured to a level where it is able to assess the complexity of the human proteome. We discuss some of the expected challenges ahead and promising strategies for success.

MeSH terms

Databases, Protein
Humans
Mass Spectrometry / trends*
Proteome / analysis*
Proteomics / methods*
Quality Control
Reproducibility of Results
Sensitivity and Specificity

Substances

Proteome