Abstract
We recently demonstrated that vaccinated rhesus macaques controlled viral replication of a heterologous SIV challenge. Here, we analyzed anamnestic SIV-specific CD4+ T-cell responses expanding immediately after challenge and show that successful vaccinees consistently targeted a short region of the Gag-p27 Capsid (amino acids 249-291). We have also defined the major histocompatibility complex class II (MHC-II) restricting alleles for several of these responses and show that DQ-restricted CD4+ T-cells depend on unique combinations of both the DQA and DQB alleles. Analysis of SIV-specific CD4+ T-cell responses elicited by a successful vaccine may have important implications in the understanding of vaccine design.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Animals
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CD4-Positive T-Lymphocytes / immunology*
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Epitopes / immunology
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Gene Products, gag / immunology*
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Genes, MHC Class II
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Histocompatibility Antigens Class II / immunology
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Immunologic Memory
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Macaca mulatta / genetics
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Macaca mulatta / immunology*
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Molecular Sequence Data
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Peptide Fragments / immunology
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Retroviridae Proteins / immunology
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SAIDS Vaccines / immunology*
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Simian Acquired Immunodeficiency Syndrome / immunology
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Simian Acquired Immunodeficiency Syndrome / prevention & control
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Simian Immunodeficiency Virus / immunology*
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Simian Immunodeficiency Virus / isolation & purification
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Simian Immunodeficiency Virus / physiology
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T-Lymphocyte Subsets / immunology*
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Vaccination
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Viral Load
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Viremia / immunology
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Viremia / prevention & control
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Virus Replication
Substances
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Epitopes
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Gag protein p27, Simian immunodeficiency virus
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Gene Products, gag
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Histocompatibility Antigens Class II
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Peptide Fragments
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Retroviridae Proteins
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SAIDS Vaccines