Abstract
This unit covers genetic manipulation protocols for M. abscessus, including nucleic acid extraction (plasmid DNA, genomic DNA, and RNA), transformation, and a recombineering mutagenesis method. M. abscessus is a Biosafety Level 2 (BSL-2) bacterium, and working considerations are also discussed.
MeSH terms
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DNA, Bacterial / genetics
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DNA, Bacterial / isolation & purification
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Genetic Engineering / methods*
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Genetics, Microbial / methods*
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Mutagenesis
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Mycobacterium / genetics*
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Plasmids
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RNA, Bacterial / genetics
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RNA, Bacterial / isolation & purification
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Recombination, Genetic
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Transformation, Bacterial
Substances
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DNA, Bacterial
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RNA, Bacterial