Abstract
A human clone corresponding to the gene for the DNA-binding factor LFB3, a protein highly homologous to the liver-specific transcription factor LFB1, has been isolated and partially sequenced. This gene is designated TCF2. Oligonucleotide primers have been designed for LFB3 and used to amplify specifically the human gene in human/rodent somatic cell hybrids using the polymerase chain reaction. By this means, the human LFB3 gene has been mapped to the long arm of chromosome 17, between the centromere and the APL breakpoint.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Chromosome Mapping / methods
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Chromosomes, Human, Pair 17*
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DNA
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DNA-Binding Proteins*
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Hepatocyte Nuclear Factor 1
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Hepatocyte Nuclear Factor 1-alpha
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Hepatocyte Nuclear Factor 1-beta
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Humans
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Hybrid Cells
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Mice
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Molecular Sequence Data
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Nuclear Proteins*
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Polymerase Chain Reaction
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Rats
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Sequence Homology, Nucleic Acid
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Transcription Factors / genetics*
Substances
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DNA-Binding Proteins
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HNF1A protein, human
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HNF1B protein, human
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Hepatocyte Nuclear Factor 1-alpha
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Hnf1a protein, mouse
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Hnf1a protein, rat
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Hnf1b protein, mouse
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Nuclear Proteins
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Transcription Factors
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Hepatocyte Nuclear Factor 1
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Hepatocyte Nuclear Factor 1-beta
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DNA