Abstract
Fluorescent proteins with emission wavelengths in the near-infrared and infrared range are in high demand for whole-body imaging techniques. Here we report near-infrared dimeric fluorescent proteins eqFP650 and eqFP670. To our knowledge, eqFP650 is the brightest fluorescent protein with emission maximum above 635 nm, and eqFP670 displays the most red-shifted emission maximum and high photostability.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Biotechnology / instrumentation
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Biotechnology / methods*
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Embryo, Nonmammalian / cytology
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Embryo, Nonmammalian / drug effects
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Embryo, Nonmammalian / metabolism
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Escherichia coli / genetics
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Escherichia coli / growth & development
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HeLa Cells
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Humans
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Infrared Rays
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Luminescent Proteins* / genetics
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Luminescent Proteins* / toxicity
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Mice
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Molecular Sequence Data
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Protein Multimerization
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Protein Stability
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Sequence Alignment
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Transfection
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Whole Body Imaging / methods*
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Xenopus laevis / genetics
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Xenopus laevis / metabolism
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Zebrafish / genetics
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Zebrafish / metabolism