A graphene-based platform for the assay of duplex-DNA unwinding by helicase

Angew Chem Int Ed Engl. 2010 Aug 2;49(33):5703-7. doi: 10.1002/anie.201001332.

Abstract

Time to unwind: Graphene oxide (GO) enables the quantitative measurement of helicase‐dependent double‐stranded DNA (dsDNA) unwinding activity in real time. GO selectively binds to unwound fluorescent‐dye‐labeled single‐stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques
  • Carbon / chemistry*
  • Coronavirus / enzymology
  • DNA / metabolism*
  • DNA Helicases / metabolism*
  • Fluorescent Dyes / chemistry
  • Nucleic Acid Denaturation
  • Oxides / chemistry
  • Spectrometry, Fluorescence

Substances

  • Fluorescent Dyes
  • Oxides
  • Carbon
  • DNA
  • DNA Helicases