Abstract
Lentiviral vector containing the HSV1-tk and firefly luciferase (fLuc) gene was infected into C6 and C6-TL expressing HSV1-tk and fLuc gene was generated. C6-TL showed higher [(125)I]IVDU uptake than C6. The survival rate of C6-TL decreased more rapidly with increasing GCV dose and was well correlated with fLuc activity. The images of microPET clearly demonstrated higher uptake of [(18)F]FHBG into the C6-TL tumor. Inhibition of tumor growth was observed in C6-TL tumor-bearing mice treated with GCV through tumor size measurement and bioluminescence imaging. The therapeutic effect of HSV1-tk/GCV system can be monitored using bioluminescent imaging and tumor size measurement.
2010 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / pharmacology*
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Brain Neoplasms / enzymology
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Brain Neoplasms / genetics
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Brain Neoplasms / therapy*
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Cell Line, Tumor
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Cell Survival / drug effects
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Dose-Response Relationship, Drug
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Female
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Ganciclovir / metabolism
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Ganciclovir / pharmacology*
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Genes, Reporter
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Genes, Transgenic, Suicide*
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Genetic Therapy / methods*
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Genetic Vectors / genetics
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Glioblastoma / enzymology
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Glioblastoma / genetics
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Glioblastoma / therapy*
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Herpesvirus 1, Human / enzymology*
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Herpesvirus 1, Human / genetics
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Idoxuridine / analogs & derivatives
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Idoxuridine / metabolism
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Kinetics
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Lentivirus / genetics
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Luciferases, Firefly / biosynthesis
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Luciferases, Firefly / genetics
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Luminescent Measurements*
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Positron-Emission Tomography
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Rats
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Recombinant Fusion Proteins / metabolism
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Thymidine Kinase / biosynthesis*
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Thymidine Kinase / genetics
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Tumor Burden / drug effects
Substances
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Antineoplastic Agents
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Recombinant Fusion Proteins
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5-(2-iodovinyl)-2'-deoxyuridine
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Luciferases, Firefly
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Thymidine Kinase
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Idoxuridine
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Ganciclovir