iPSC lines that do not silence the expression of the ectopic reprogramming factors may display enhanced propensity to genomic instability

Cell Res. 2010 Oct;20(10):1092-5. doi: 10.1038/cr.2010.125. Epub 2010 Sep 7.

Abstract

Here, we provide data suggesting that the absence of silencing of the ectopic reprogramming factors used to reprogram somatic cells to induced pluripotent stem cells (iPSCs) may predispose iPSCs to genomic instability. We encourage stem cell scientists to undertake an extensive characterization and standardization of much larger cohorts of iPSC lines in order to set up rigorous criteria to define safe and stable bona fide iPSCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cellular Reprogramming*
  • Gene Silencing
  • Genomic Instability*
  • Induced Pluripotent Stem Cells / metabolism*
  • Karyotyping
  • Lentivirus / genetics