The detailed synthetic protocol for a 2-selenothymidine phosphoramidite and its use in preparing Se-derivatized oligonucleotides are described here. The Se-modified phosphoramidite synthesis was achieved by activating a 2-thiothymidine derivative, followed by introduction of selenium functionality. The coupling reaction yield of the 2-selenothymidine phosphoramidite during solid-phase synthesis is high (>95%), and the oligonucleotides containing the 2-selenothymidine derivatization are stable.
Copyright 2010 by John Wiley & Sons, Inc.