Abstract
This study was aimed to test a panel of six housekeeping genes (GAPDH, HPRT1, POLR2A, RPLP0, ACTB, and H3F) so as to identify and validate the most suitable reference genes for expression studies in astrocytomas. GAPDH was the most stable and HPRT1 was the least stable reference gene. The effect of reference gene selection on quantitative real-time polymerase chain reaction data interpretation was demonstrated, normalizing the expression data of a selected gene of interest. Thus, GAPDH may be recommended for data normalization in gene expression studies in astrocytomas. Nevertheless, a preliminary validation of reference gene stability is required prior to every study.
Copyright © 2010 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / genetics
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Actins / metabolism
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Actins / standards
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Astrocytoma / genetics
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Astrocytoma / metabolism*
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Gene Expression Profiling*
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Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / genetics
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Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / metabolism
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Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / standards
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Histones / genetics
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Histones / metabolism
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Histones / standards
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Humans
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Hypoxanthine Phosphoribosyltransferase / genetics
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Hypoxanthine Phosphoribosyltransferase / metabolism
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Hypoxanthine Phosphoribosyltransferase / standards
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / standards
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Reference Standards
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Ribosomal Proteins / genetics
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Ribosomal Proteins / metabolism
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Ribosomal Proteins / standards
Substances
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Actins
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Histones
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Ribosomal Proteins
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Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
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Hypoxanthine Phosphoribosyltransferase