Lipid bilayer domain fluctuations as a probe of membrane viscosity

Biophys J. 2010 Sep 22;99(6):L44-6. doi: 10.1016/j.bpj.2010.07.007.

Abstract

We argue that membrane viscosity, η(m), plays a prominent role in the thermal fluctuation dynamics of micron-scale lipid domains. A theoretical expression is presented for the timescales of domain shape relaxation, which reduces to the well-known η(m) = 0 result of Stone and McConnell in the limit of large domain sizes. Experimental measurements of domain dynamics on the surface of ternary phospholipid and cholesterol vesicles confirm the theoretical results and suggest domain flicker spectroscopy as a convenient means to simultaneously measure both the line tension, σ, and the membrane viscosity, η(m), governing the behavior of individual lipid domains.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Membrane / chemistry*
  • Cell Membrane / metabolism
  • Hydrodynamics
  • Lipid Bilayers / chemistry*
  • Lipid Bilayers / metabolism
  • Models, Biological*
  • Spectrum Analysis
  • Time Factors
  • Viscosity

Substances

  • Lipid Bilayers