Fluorescent nucleoside analogue displays enhanced emission upon pairing with guanine

Org Biomol Chem. 2010 Nov 21;8(22):5053-5. doi: 10.1039/c0ob00413h. Epub 2010 Sep 23.

Abstract

A fluorescent nucleobase analogue, 7-aminoquinazoline-2,4-(1H,3H)-dione, is incorporated into a DNA oligonucleotide and senses mismatched pairing by displaying G-specific fluorescence enhancement.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Guanine / chemistry*
  • Molecular Sequence Data
  • Nucleosides / chemical synthesis
  • Nucleosides / chemistry*
  • Oligonucleotides / genetics
  • Optical Phenomena
  • Spectrometry, Fluorescence

Substances

  • Nucleosides
  • Oligonucleotides
  • Guanine