TEM image processing tools are devised for the assessment of 2D-crystallization experiments. The algorithms search for the presence and assess the quality of crystalline membranes. The retained scenario emulates the decisions of a microscopist in selecting targets and assessing the sample. Crystallinity is automatically assessed through the diffraction patterns of high magnification images acquired on pertinent regions selected at lower magnifications. Further algorithms have been developed for membrane characterization. Tests on images of different samples, acquired on different microscopes led to good results.