Abstract
Class IIa bacteriocins target a phylogenetically defined subgroup of mannose-phosphotransferase systems (man-PTS) on sensitive cells. By the use of man-PTS genes of the sensitive Listeria monocytogenes (mpt) and the nonsensitive Lactococcus lactis (ptn) species to rationally design a series of man-PTS chimeras and site-directed mutations, we identified an extracellular loop of the membrane-located protein MptC that was responsible for specific target recognition by the class IIa bacteriocins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antibiosis*
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Bacterial Proteins / antagonists & inhibitors*
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Bacteriocins / metabolism
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Bacteriocins / pharmacology*
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Lactococcus lactis / drug effects
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Lactococcus lactis / enzymology*
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Listeria monocytogenes / drug effects
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Listeria monocytogenes / enzymology*
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Mutant Proteins / genetics
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Mutant Proteins / metabolism
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Phosphoenolpyruvate Sugar Phosphotransferase System / antagonists & inhibitors*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Recombination, Genetic
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Sequence Alignment
Substances
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Bacterial Proteins
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Bacteriocins
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Mutant Proteins
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Recombinant Proteins
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Phosphoenolpyruvate Sugar Phosphotransferase System
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phosphoenolpyruvate-mannose phosphotransferase