Abstract
Four Streptococcus pneumoniae isolates expressing both 6A and 6B capsular serotypes were detected by a multiplex immunoassay. The sequence of WciP, a GT2-family glycosyltransferase, indicates that point mutation has compromised linkage specificity, allowing two alternative oligosaccharides to be synthesized. This finding highlights that mutation as well as recombination can mediate serotype change.
MeSH terms
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Bacterial Capsules / immunology*
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Bacterial Capsules / metabolism*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Epitopes / immunology*
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Gene Expression
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Glycosyltransferases / genetics*
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Glycosyltransferases / metabolism*
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Molecular Sequence Data
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Mutation, Missense
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Oligosaccharides / metabolism
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Sequence Analysis, DNA
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Serotyping
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Streptococcus pneumoniae / genetics*
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Streptococcus pneumoniae / immunology*
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Substrate Specificity
Substances
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Bacterial Proteins
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DNA, Bacterial
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Epitopes
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Oligosaccharides
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Glycosyltransferases
Associated data
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GENBANK/HQ260721
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GENBANK/HQ260722
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GENBANK/HQ260723
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GENBANK/HQ260724
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GENBANK/HQ260725