Rheumatoid arthritis and salivary biomarkers of periodontal disease

J Clin Periodontol. 2010 Dec;37(12):1068-74. doi: 10.1111/j.1600-051X.2010.01625.x. Epub 2010 Sep 28.

Abstract

Aim: To test the hypothesis that rheumatoid arthritis (RA) influenced levels of salivary biomarkers of periodontal disease.

Methods: Medical assessments, periodontal examinations and pain ratings were obtained from 35 RA, 35 chronic periodontitis and 35 age- and gender-matched healthy controls in a cross-sectional, case-controlled study. Unstimulated whole saliva samples were analysed for interleukin-1β (IL-1β), matrix metalloproteinase-8 (MMP-8) and tumour necrosis factor-α (TNF-α) concentrations.

Results: The arthritis and healthy groups had significantly less oral disease than the periodontitis group (P<0.0001), with the arthritis group having significantly more sites bleeding on probing (BOP) than matched controls (P=0.012). Salivary levels of MMP-8 and IL-1β were significantly elevated in the periodontal disease group (P<0.002), and IL-1β was the only biomarker with significantly higher levels in the arthritis group compared with controls (P=0.002). Arthritis patients receiving anti-TNF-α antibody therapy had significantly lower IL-1β and TNF-α levels compared with arthritis patients not on anti-TNF-α therapy (P=0.016, 0.024) and healthy controls (P<0.001, P=0.011), respectively.

Conclusion: RA patients have higher levels of periodontal inflammation than healthy controls, i.e., an increased BOP. Systemic inflammation appears to influence levels of select salivary biomarkers of periodontal disease, and anti-TNF-α antibody-based disease-modifying therapy significantly lowers salivary IL-1β and TNF-α levels in RA.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Alveolar Bone Loss / metabolism
  • Antibodies, Monoclonal / therapeutic use
  • Antirheumatic Agents / therapeutic use
  • Arthritis, Rheumatoid / drug therapy
  • Arthritis, Rheumatoid / metabolism*
  • Biomarkers / analysis
  • Case-Control Studies
  • Chronic Periodontitis / metabolism*
  • Cross-Sectional Studies
  • Dental Plaque Index
  • Female
  • Gingival Hemorrhage / metabolism
  • Humans
  • Interleukin-1beta / analysis
  • Interleukin-1beta / drug effects
  • Male
  • Matrix Metalloproteinase 8 / analysis
  • Matrix Metalloproteinase 8 / drug effects
  • Middle Aged
  • Periodontal Attachment Loss / metabolism
  • Periodontal Index
  • Periodontal Pocket / metabolism
  • Radiography, Bitewing
  • Saliva / chemistry*
  • Tumor Necrosis Factor-alpha / analysis
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors
  • Young Adult

Substances

  • Antibodies, Monoclonal
  • Antirheumatic Agents
  • Biomarkers
  • Interleukin-1beta
  • Tumor Necrosis Factor-alpha
  • MMP8 protein, human
  • Matrix Metalloproteinase 8