Computational fluid dynamics modeling of momentum transport in rotating wall perfused bioreactor for cartilage tissue engineering

J Biotechnol. 2010 Nov;150(3):389-95. doi: 10.1016/j.jbiotec.2010.09.950. Epub 2010 Sep 29.

Abstract

In this study, computational fluid dynamics (CFD) analysis of a rotating-wall perfused-vessel (RWPV) bioreactor is performed to characterize the complex hydrodynamic environment for the simulation of cartilage development in RWPV bioreactor in the presence of tissue-engineered cartilage constructs, i.e., cell-chitosan scaffolds. Shear stress exerted on chitosan scaffolds in bioreactor was calculated for different rotational velocities in the range of 33-38 rpm. According to the calculations, the lateral and lower surfaces were exposed to 0.07926-0.11069 dyne/cm(2) and 0.05974-0.08345 dyne/cm(2), respectively, while upper surfaces of constructs were exposed to 0.09196-0.12847 dyne/cm(2). Results validate adequate hydrodynamic environment for scaffolds in RWPV bioreactor for cartilage tissue development which concludes the suitability of operational conditions of RWPV bioreactor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Animals
  • Bioreactors*
  • Cartilage
  • Chondrocytes
  • Computational Biology / methods*
  • Computer Simulation
  • Computer-Aided Design
  • Hydrodynamics
  • Mice
  • Reproducibility of Results
  • Tissue Engineering / instrumentation*
  • Tissue Engineering / methods
  • Tissue Scaffolds